Research reported in the following peer-reviewed journal articles relied on the Biophysics Instrumentation Facility. Please add your publication to this list by sending a PDF to Dr. Darrell McCaslin. Suggested Acknowledgment
“(Instrument) data were obtained at the University of Wisconsin – Madison Biophysics Instrumentation Facility, which was established with support from the University of Wisconsin – Madison and grants BIR-9512577 (NSF) and S10 RR13790 (NIH).”
The publications on this site are being made available to the academic community for use in teaching and research. All copyrights remain intact.
2011
Watkins, R. W., Arnold, U., and Raines, R. T. Ribonuclease S redux. Chem. Commun. 47, 973-975.
2010
Chao, T.-Y., Lavis, L. D., Raines, R. T. (2010) Cellular uptake of ribonuclease A relies on anionic glycans. Biochemistry 49, 10666-10673.
Krow, G. R., Liu, N., Sender, M., Lin, G., Centafont, R., Sonnet, P. E., DeBrosse, C., Ross, C. W., III, Carroll, P. J., Shoulder, M. D., and Raines, R. T. Org. Lett. 12, 5438-5441.
Rutkoski, T. J., Kink, J. A., Strong, L. E., Schilling, C. I., and Raines, R. T. (2010) Antitumor activity of ribonuclease multimers created by site-specific covalent tethering. Bioconjugate Chem. 21, 1691-1702.
Shoulders, M. D., Satyshur, K. A., Forest, K. T., and Raines, R. T. (2010) Stereoelectronic and steric effect in side chains preorganize a protein main chain. Proc. Natl. Acad. Sci. USA 107, 559-564.
2009
Atapattu, D. N., Aulik, N. A., McCaslin, D. R., and Czuprynski, C. J. (2009) Brief heat treatment increases cytotoxicity of Mannheimia haemolytica leukotoxin in an LFA-1 independent manner. Microb. Pathog. 46, 159-165.
Gorres, K. L., Pua, K. H., and Raines, R. T. (2009) Stringency of the 2-His – 1-Asp active-site motif in prolyl 4-hydroxylase. PLoS ONE 4, e7635.
Shoulders, M. D., Kamer, K. J., and Raines, R. T. (2009) Origin of the stability conferred upon collagen by fluorination. Bioorg. Med. Chem. Lett. 19, 3859-3862.
Turcotte, R. F., Lavis, L. D., and Raines, R. T. (2009) Onconase cytotoxicity relies on the distribution of its positive charge. FEBS J. 276, 4270-4281.
2008
Brunzelle, J. S., Jordan, D. B., McCaslin, D. R., Olczak, A., and Wawrzak, Z. (2008) Structure of the two-subsite beta-D-xylosidase from Selenomonas ruminantium in complex with 1,3-bis[tris(hydroxymethyl)methylamino]propane. Arch. Biochem. Biophys. 474, 157-166.
Gorres, K. L., Edupuganti, R., Krow, G. R., Raines, R. T. (2008) Conformational preferences of substrates for prolyl 4-hydroxylase. Biochemistry 47, 9447-9455.
Horne, W. S., Price, J. L., and Gellman, S. H. (2008) Interplay among side chain sequence, backbone composition, and residue rigidification in polypeptide folding and assembly. Proc. Natl. Acad. Sci. USA 105, 9151-9156.
Turcotte, R., and Raines, R. T. (2008) Design and characterization of an HIV-specific ribonuclease zymogen. AIDS Res. Human Retroviruses 24, 1357-1363.
Levine, M. N., Lavis, L. D., and Raines, R. T. (2008) Trimethyl lock: A stable chromogenic substrate for esterases. Molecules 13, 204-211.
2007
Lavis, L. D., Rutkoski, T. J., and Raines, R. T. (2007) Tuning the pK(a) of fluorescein to optimize binding assays. Anal. Chem. 79, 6775-6782.
Johnson, R. J., Lin, S. R., and Raines, R. T. (2007) Genetic selection reveals the role of a buried, conserved polar residue. Protein Sci. 16, 1609-1616.
Fuchs, S. M., Rutkoski, T. J., Kung, V. M., Groeschl, R. T., and Raines, R. T. (2007) Increasing the potency of a cytotoxin with an arginine graft. Protein Eng. Des. Select. 20, 505-509.
Johnson, R. J., McCoy, J. G., Bingman, C. A., Phillips, G. N., Jr., and Raines, R. T. (2007) Inhibition of human pancreatic ribonuclease by the human ribonuclease inhibitor protein. J. Mol. Biol. 368, 434-449.
Schechter, N. M., Choi, E. J., Selwood, T., and McCaslin, D. T. (2007) Characterization of three distinct catalytic forms of human tryptase-beta: Their interrelationships and relevance. Biochemistry 46, 9615-9629.
2006
Drees, J. C., Chitteni-Pattu, S., McCaslin, D. R., Inman, R. B., and Cox, M. M. (2006) Inhibition of RecA protein function by the RdgC protein from Escherichia coli. J. Biol. Chem. 281, 4708-4717. [PDF]
Hodges, J. A., and Raines, R. T. (2006) Energetics of an n->π* interaction that impacts protein structure. Org. Lett. 8, 4695-4697. [PDF]
Horng, J. C., Hawk, A. J., Zhao, Q., Benedict, E. S., Burke, S. D., and Raines R. T. (2006) Macrocyclic scaffold for the collagen triple helix. Org. Lett. 8, 4735-4738. [PDF]
Horng, J. C., and Raines, R. T. (2006) Stereoelectronic effects on polyproline conformation. Protein Sci. 15, 74-83. [PDF]
Kotch, F. W., and Raines, R. T. (2006) Self-assembly of synthetic collagen triple helices. Proc. Natl. Acad. Sci. USA 103, 3028-3033. [PDF]
Johnson, R. J., Lin, S. R., and Raines, R. T. (2006) A ribonuclease zymogen activated by the NS3 protease of the hepatitis C virus. FEBS J. 273, 5457-5465. [PDF]
Shoulders, M. D., Hodges, J. A., and Raines, R. T. (2006) Reciprocity of steric and stereoelectronic effects in the collagen triple helix. J. Am. Chem. Soc. 128, 8112-8113. [PDF]
Smith, B. D., and Raines, R. T. (2006) Genetic selection for critical residues in ribonucleases. J. Mol. Biol. 362, 459-478. [PDF]
2005
Dye, B. T., Miller, D. J., and Ahlquist, P. (2005) In vivo self-interaction of nodavirus RNA replicase protein A revealed by fluorescence resonance energy transfer. J. Virol. 79, 8909-8919. [PDF]
Hodges, J. A., and Raines, R. T. (2005) Stereoelectronic and steric effects in the collagen triple helix: Toward a code for strand association. J. Am. Chem. Soc. 127, 15923-15932. [PDF]
Kersteen, E. A., Barrows, S. R., and Raines, R. T. (2005) Catalysis of protein disulfide bond isomerization in a homogeneous substrate. Biochemistry 44, 12168-12178. [PDF]
Miller, B. G., and Raines, R. T. (2005) Reconstitution of a defunct glycolytic pathway via recruitment of ambiguous sugar kinases. Biochemistry 44, 10776-10783. [PDF]
Rutkoski, T. J., Kurten, E. L., Mitchell, J. C., and Raines, R. T. (2005) Disruption of shape-complementarity markers to create cytotoxic variants of ribonuclease A. J. Mol. Biol. 354, 41-54. [PDF]
Selwood, T., Smolensky, H., McCaslin, D. R., and Schechter, N. M. (2005) The interaction of human tryptase-beta with small molecule inhibitors provides new insights into the unusual functional instability and quaternary structure of the protease. Biochemistry 44, 3580-3590. [PDF]
2004
Eggington, J. M., Haruta, N., Wood, E. A., and Cox, M. M. (2004) The single-stranded DNA-binding protein of Deinococcus radiodruans. BMC Microbiol. 4, 1-12. [PDF]
Miller, B. G., and Raines, R. T. (2004) Identifying latent enzyme activities: Substrate ambiguity within modern bacterial sugar kinases. Biochemistry 43, 6387-6392. [PDF]
Rubio, L. M., Singer, S. W., and Ludden, P. W. (2004) Purification and characterization of NafY (apodinitrogenase gamma subunit) from Azotobacter vinelandii. J. Biol. Chem. 279, 19739-19746. [PDF]
Subramanian, L., Crabb, J. W., Cox, J., Durussel, I., Walker, T. M., van Ginkel, P. R., Bhattacharya, S., Dellaria, J. M., Palczewski, K., and Polans, A. S. (2004) Ca2+ binding to EF Hands 1 and 3 is essential for the interaction of apoptosis-linked gene-2 with Alix/AIP1 in ocular melanoma. Biochemistry 43, 11175-11186. [PDF]
2003
Felitsky, D. J., and Record, M. T., Jr. (2003) Thermal and urea-induced unfolding of the marginally stable lac repressor DNA-binding domain: A model system for analysis of solute effects on protein processes. Biochemistry 42, 2202-2217. [PDF]
Hannah, B. L., Misenheimer, T. M., Annis, D. S., and Mosher, D. F. (2003) A polymorphism in thrombospondin-1 associated with familial premature coronary heart disease causes a local change in conformation of the Ca2+-binding repeats. J. Biol. Chem. 278, 8929-8934. [PDF]
Hinderaker, M. P., and Raines, R. T. (2003) An electronic effect on protein structure. Protein Sci. 12, 1188-1194. [PDF]
Hodges, J. A., and Raines, R. T. (2003) Stereoelectronic effects on collagen stability: The dichotomy of 4-fluoroproline diastereomers. J. Am. Chem. Soc. 125, 9262-9263. [PDF]
Jenkins, C. L., Bretscher, L. E., Guzei, I. A., and Raines, R. T. (2003) Effect of 3-hydroxyproline residues on collagen stability. J. Am. Chem. Soc. 125, 6422-6427. [PDF]
Lai, J. R., and Gellman, S. H. (2003) Reinvestigation of the proposed folding and self-association of the neuropeptide head activator. Protein Sci. 12, 560-566. [PDF]
Lee, J. E., and Raines, R. T. (2003) Contribution of active-site residues to the function of onconase, a ribonuclease with antitumoral activity. Biochemistry 42, 11443-11450. [PDF]
Misenheimer, T. M., Hannah, B. L., Annis, D. S., and Mosher, D. F. (2003) Interactions among the three structural motifs of the C-terminal region of human thrombospondin-2. Biochemistry 42, 5125-5132. [PDF]
Park, J. S., Lee, H. S., Lai, J. R., Kim, B. M., and Gellman, S. H. (2003) Accommodation of alpha-substituted residues in the beta-peptide 12-helix: Expanding the range of substitution patterns available to a foldamer scaffold. J. Am. Chem. Soc. 125, 8539-8545. [PDF]
Raguse, T. L., Lai, J. R., and Gellman, S. H. (2003) Environment-independent 14-helix formation in short beta-peptides: Striking a balance between shape control and functional diversity. J. Am. Chem. Soc. 125, 5592-5593. [PDF]
Schinnerl, M., Murray, J., Langenhan, J. M., and Gellman, S. H. (2003) Asymmetric synthesis of a new helix-forming beta-amino acid: trans-4-Aminopiperidine-3-carboxylic acid. Eur. J. Org. Chem., 721-726. [PDF]
Sheehan, J. P., Kobbervig, C. E., and Kirkpatrick, H. M. (2003) Heparin inhibits the intrinsic tenase complex by interacting with an exosite on factor IXa. Biochemistry 42, 11316-11325. [PDF]
Syud, F. A., Stanger, H. E., Mortell, H. S., Espinosa, J. F., Fisk, J. D., Fry, C. G., and Gellman, S. H. (2003) Influence of strand number on antiparallel beta-sheet stability in designed three- and four-stranded beta-sheets. J. Mol. Biol. 326, 553-568. [PDF]
2002
Cairo, C. W., Strzelec, A., Murphy, R. M., and Kiessling, L. L. (2002) Affinity-based inhibition of beta-amyloid toxicity. Biochemistry 41, 8620-8629. [PDF]
Espinosa, J. F., Syud, F. A., and Gellman, S. H. (2002) Analysis of the factors that stabilize a designed two-stranded antiparallel beta-sheet. Protein Sci. 11, 1492-1505. [PDF]
Gestwicki, J. E., Cairo, C. W., Mann, D. A., Owen, R. M., and Kiessling, L. L. (2002) Selective immobilization of multivalent ligands for surface plasmon resonance and fluorescence microscopy. Anal. Biochem. 305, 149-155. [PDF]
Hegeman, A. D., Gross, J. W., and Frey, P. A. (2002) Concerted and stepwise dehydration mechanisms observed in wild-type and mutated Escherichia coli dTDP-glucose 4,6-dehydratase. Biochemistry 41, 2797-2804. [PDF]
Herdendorf, T. J., McCaslin, D. R., and Forest, K. T. (2002) Aquifex aeolicus PilT, homologue of a surface motility protein, is a thermostable oligomeric NTPase. J. Bacteriol. 184, 6465-6471. [PDF]
Huwiler, K. G., Vestling, M. M., Annis, D. S., and Mosher, D. F. (2002) Biophysical characterization, including disulfide bond assignments, of the anti-angiogenic type 1 domains of human thrombospondin-1. Biochemistry 41, 14329-14339. [PDF]
Kuloglu, E. S., McCaslin, D. R., Markley, J. L., and Volkman, B. F. (2002) Structural rearrangement of human lymphotactin, a C chemokine, under physiological solution conditions. J. Biol. Chem. 277, 17863-17870. [PDF]
Lai, J. R., Huck, B. R., Weisblum, B., and Gellman, S. H. (2002) Design of non-cysteine-containing antimicrobial beta-hairpins: Structure?activity relationship studies with linear protegrin-1 analogues. Biochemistry 41, 12835-12842. [PDF]
LePlae, P. R., Fisk, J. D., Porter, E. A., Weisblum, B., and Gellman, S. H. (2002) Tolerance of acyclic residues in the beta-peptide 12-helix: Access to diverse side-chain arrays for biological applications. J. Am. Chem. Soc. 124, 6820-6821. [PDF]
Porter, E. A., Wang, X., Schmitt, M. A., and Gellman, S. H. (2002) Synthesis and 12-helical secondary structure of beta-peptides containing (2R,3R)-aminoproline. Org. Lett. 4, 3317-3319. [PDF]
Porter, E. A., Weisblum, B., and Gellman, S. H. (2002) Mimicry of host-defense peptides by unnatural oligomers: Antimicrobial beta-peptides. J. Am. Chem. Soc. 124, 7324-7330. [PDF]
Raguse, T. R., Lai, J. R., and Gellman, S. H. (2002a) Evidence that the beta-peptide 14-helix is stabilized by beta3-residues with sidechain branching adjacent to the beta-carbon. Helv. Chim. Acta 85, 4154-4164. [PDF]
Raguse, T. L., Porter, E. A., Weisblum, B., and Gellman, S. H. (2002b) Structure?activity studies of 14-helical antimicrobial beta-peptides: Probing the relationship between conformational stability and antimicrobial potency. J. Am. Chem. Soc. 124, 12774-12785. [PDF]
Selwood, T., Wang, Z. M., McCaslin, D. R., and Schechter, N. M. (2002) Diverse stability and catalytic properties of human tryptase alpha and beta isoforms are mediated by residue differences at the S1 pocket. Biochemistry 41, 3329-3340. [PDF]
Woll, M. G., Fisk, J. D., LePlae, P. R., and Gellman, S. H. (2002) Stereoselective synthesis of 3-substituted 2-aminocyclopentanecarboxylic acid derivatives and their incorporation into short 12-helical beta-peptides that fold in water. J. Am. Chem. Soc. 124, 12447-12452. [PDF]
2001
Bretscher, L. E., Jenkins, C. L., Taylor, K. M., DeRider, M. L., and Raines, R. T. (2001) Conformational stability of collagen relies on a stereoelectronic effect. J. Am. Chem. Soc. 123, 777-778. [PDF]
Espinosa, J. F., Munoz, V., and Gellman, S. H. (2001) Interplay between hydrophobic cluster and loop propensity in beta-hairpin formation. J. Mol. Biol. 306, 397-402. [PDF]
Fisk, J. D., and Gellman, S. H. (2001) A parallel beta-sheet model system that folds in water. J. Am. Chem. Soc. 123, 343-344. [PDF]
Gross, J. W., Hegeman, A. D., Gerratana, B., and Frey, P. A. (2001) Dehydration is catalyzed by glutamate-136 and aspartic acid-135 active site residues in Escherichia coli dTDP-glucose 4,6-dehydratase. Biochemistry 40, 12497-12504. [PDF]
Hegeman, A. D., Gross, J. W., and Frey, P. A. (2001) Probing catalysis by Escherichia coli dTDP-glucose-4,6-dehydratase: Identification and preliminary characterization of functional amino acid residues at the active site. Biochemistry 40, 6598-6610. [PDF]
Isenbarger, T. A., and Krebs, M. P. (2001) Thermodynamic stability of the bacteriorhodopsin lattice as measured by lipid dilution. Biochemistry 40, 11923-11931. [PDF]
Kersteen, E. A., and Raines, R. T. (2001) Contribution of tertiary amides to the conformational stability of collagen triple helices. Biopolymers 59, 24-28. [PDF]
Klink, T. A., Vicentini, A. M., Hofsteenge, J., and Raines, R. T. (2001) High-level soluble production and characterization of porcine ribonuclease inhibitor. Protein Expr. Purif. 22, 174-179. [PDF]
Kuloglu, E. S., McCaslin, D. R., Kitabwalla, M., Pauza, C. D., Markley, J. L., and Volkman, B. F. (2001) Monomeric solution structure of the prototypical ‘C’ chemokine lymphotactin. Biochemistry 40, 12486-12496. [PDF]
Lee, H. S., Syud, F. A., Wang, X., and Gellman, S. H. (2001) Diversity in short beta-peptide 12-helices: High-resolution structural analysis in aqueous solution of a hexamer containing sulfonylated pyrrolidine residues. J. Am. Chem. Soc. 123, 7721-7722. [PDF]
Misenheimer, T. M., Hahr, A. J., Harms, A. C., Annis, D. S., and Mosher, D. F. (2001) Disulfide connectivity of recombinant C-terminal region of human thrombospondin 2. J. Biol. Chem. 276, 45882-45887. [PDF]
Park, C., Schultz, L. W., and Raines, R. T. (2001) Contribution of the active site histidine residues of ribonuclease A to nucleic acid binding. Biochemistry 40, 4949-4956. [PDF]
Raguse, T. L., Lai, J. R., LePlae, P. R., and Gellman, S. H. (2001) Toward beta-peptide tertiary structure: Self-association of an amphiphilic 14-helix in aqueous solution. Org. Lett. 3, 3963-3966. [PDF]
Stanger, H. E., Syud, F. A., Espinosa, J. F., Giriat, I., Muir, T., and Gellman, S. H. (2001) Length-dependent stability and strand length limits in antiparallel beta-sheet secondary structure. Proc. Natl. Acad. Sci. USA 98, 12015-12020. [PDF]
Syud, F. A., Stanger, H. E., and Gellman, S. H. (2001) Interstrand side chain?side chain interactions in a designed beta-hairpin: Significance of both lateral and diagonal pairings. J. Am. Chem. Soc. 123, 8667-8677. [PDF]
2000
Espinosa, J. F., and Gellman, S. H. (2000) A designed beta-hairpin containing a natural hydrophobic cluster. Angew. Chem. Int. Ed. Eng. 39, 2330-2333. [PDF]
Klink, T. A., and Raines, R. T. (2000) Conformational stability is a determinant of ribonuclease A cytotoxicity. J. Biol. Chem. 275, 17463-17467. [PDF]
Klink, T. A., Woycechowsky, K. J., Taylor, K. M., and Raines, R. T. (2000) Contribution of disulfide bonds to the conformational stability and catalytic activity of ribonuclease A. Eur. J. Biochem. 267, 566-572. [PDF]
Lee, L. V., Poyner, R. R., Vu, M. V., and Cleland, W. W. (2000) Role of metal ions in the reaction catalyzed by L-ribulose-5-phosphate 4-epimerase. Biochemistry 39, 4821-4830. [PDF]
Lee, L. V., Vu, M. V., and Cleland, W. W. (2000) 13C and deuterium isotope effects suggest an aldol cleavage mechanism for L-ribulose-5-phosphate 4-epimerase. Biochemistry 39, 4808-4820. [PDF]
Leland, P. A., Staniszewski, K. E., Kim, B., and Raines, R. T. (2000) A synapomorphic disulfide bond is critical for the conformational stability and cytotoxicity of an amphibian ribonuclease. FEBS Lett. 477, 203-207. [PDF]
McQuade, D. T., Quinn, M. A., Yu, S. M., Polans, A. S., Krebs, M. P., and Gellman, S. H. (2000) Rigid amphiphiles for membrane protein manipulation. Angew. Chem. Int. Ed. Eng. 39, 758-761. [PDF]
Messmore, J. M., and Raines, R. T. (2000) Decavanadate inhibits catalysis by ribonuclease A. Arch. Biochem. Biophys. 381, 25-30. [PDF]
Messmore, J. M., and Raines, R. T. (2000) Pentavalent organo-vanadates as transition state analogues for phosphoryl transfer reactions. J. Am. Chem. Soc. 122, 9911-9916. [PDF]
Misenheimer, T. M., Huwiler, K. G., Annis, D. S., and Mosher, D. F. (2000) Physical characterization of the procollagen module of human thrombospondin 1 expressed in insect cells. J. Biol. Chem. 275, 40938-40945. [PDF]
Wang, X., Espinosa, J. F., and Gellman, S. H. (2000) 12-Helix formation in aqueous solution with short beta-peptides containing pyrrolidine-based residues. J. Am. Chem. Soc. 122, 4821-4822. [PDF]
Yu, S. M., McQuade, D. T., Quinn, M. A., Hackenberger, C. P., Krebs, M. P., Polans, A. S., and Gellman, S. H. (2000) An improved tripod amphiphile for membrane protein solubilization. Protein Sci. 9, 2518-2527. [PDF]
1999
Amann, K. J., Guo, A. W., and Ervasti, J. M. (1999) Utrophin lacks the rod domain actin binding activity of dystrophin. J. Biol. Chem. 274, 35375-35380. [PDF]
Appella, D. H., Christianson, L. A., Karle, I. L., Powell, D. R., and Gellman, S. H. (1999) Synthesis and characterization of trans-2-aminocyclohexanecarboxylic acid oligomers: An unnatural secondary structure and implications for beta-peptide tertiary structure. J. Am. Chem. Soc. 121, 6206-6212. [PDF]
Appella, D. H., Christianson, L. A., Klein, D. A., Richards, M. R., Powell, D. R., and Gellman, S. H. (1999) Synthesis and characterization of helix-forming beta-peptides: trans-2-Aminocyclopentanecarboxylic acid oligomers. J. Am. Chem. Soc. 121, 7574-7581. [PDF]
Daugherty, D. L., and Gellman, S. H. (1999) A fluorescence assay of leucine zipper dimerization: Avoiding unintended consequences of fluorophore attachment. J. Am. Chem. Soc. 121, 4325-4333. [PDF]
Geeganage, S., and Frey, P. A. (1999) Significance of metal ions in galactose-1-phosphate uridylyltransferase: an essential structural zinc and a nonessential structural iron. Biochemistry 38, 13398-13406. [PDF]
Holmgren, S. K., Bretscher, L. E., Taylor, K. M., and Raines, R. T. (1999) A hyperstable collagen mimic. Chem. Biol. 6, 63-70. [PDF]
Huck, B. R., Langenhan, J. M., and Gellman, S. H. (1999) Non-hydrogen-bonded secondary structure in beta-peptides: Evidence from circular dichroism of (S)-pyrrolidine-3-carboxylic acid oligomers and (S)-nipecotic acid oligomers. Org. Lett. 1, 1717-1720. [PDF]
Isenbarger, T. A., and Krebs, M. P. (1999) Role of helix?helix interactions in assembly of the bacteriorhodopsin lattice. Biochemistry 38, 9023-9030. [PDF]
Mannino, S. J., Jenkins, C. L., and Raines, R. T. (1999) Chemical mechanism of DNA cleavage by the homing endonuclease I-PpoI. Biochemistry 38, 16178-16186. [PDF]
Ralph, J., Peng, J., Lu, F., Hatfield, R. D., and Helm, R. F. (1999) Are lignins optically active? J. Agric. Food Chem. 47, 2991-2996. [PDF]
Strugnell, S. A., Hill, J. J., McCaslin, D. R., Wiefling, B. A., Royer, C. A., and DeLuca, H. F. (1999) Bacterial expression and characterization of the ligand-binding domain of the vitamin D receptor. Arch. Biochem. Biophys. 364, 42-52. [PDF]
Sukharev, S. I., Schroeder, M. J., and McCaslin, D. R. (1999) Stoichiometry of the large conductance bacterial mechanosensitive channel of E. coli. A biochemical study. J. Membr. Biol. 171, 183-193. [PDF]
Syud, F. A., Espinosa, J. F., and Gellman, S. H. (1999) NMR-Based quantification of beta-sheet populations in aqueous solution through use of reference peptides for the folded and unfolded states. J. Am. Chem. Soc. 121, 11577-11578. [PDF]
1998
Amann, K. J., Renley, B. A., and Ervasti, J. M. (1998) A cluster of basic repeats in the dystrophin rod domain binds F-actin through an electrostatic interaction. J. Biol. Chem. 273, 28419-28423. [PDF]
Fisher, B. M., Schultz, L. W., and Raines, R. T. (1998) Coulombic effects of remote subsites on the active site of ribonuclease A. Biochemistry 37, 17386-17401. [PDF]
Geeganage, S., and Frey, P. A. (1998) Transient kinetics of formation and reaction of the uridylyl-enzyme form of galactose-1-P uridylyltransferase and its Q168R-variant: insight into the molecular basis of galactosemia. Biochemistry 37, 14500-14507. [PDF]
Holmgren, S. K., Taylor, K. M., Bretscher, L. E., and Raines, R. T. (1998) Code for collagen’s stability deciphered. Nature 392, 666-667. [PDF]
Leland, P. A., Schultz, L. W., Kim, B. M., and Raines, R. T. (1998) Ribonuclease A variants with potent cytotoxic activity. Proc. Natl. Acad. Sci. USA 95, 10407-10412. [PDF]
Mahnke Braam, L. A., and Reznikoff, W. S. (1998) Functional characterization of the Tn5 transposase by limited proteolysis. J. Biol. Chem. 273, 10908-10913. [PDF]
Mann, D. A., Kanai, M., Maly, D. J., and Kiessling, L. L. (1998) Probing low affinity and multivalent interactions with surface plasmon resonance: Ligands for concanavalin A. J. Am. Chem. Soc. 120, 10575-10582. [PDF]
Schenck, H. L., and Gellman, S. H. (1998) Use of a designed triple-stranded antiparallel beta-sheet to probe beta-sheet cooperativity in aqueous solution. J. Am. Chem. Soc. 120, 4869-4870. [PDF]
Schultz, L. W., Hargraves, S. R., Klink, T. A., and Raines, R. T. (1998) Structure and stability of the P93G variant of ribonuclease A. Protein Sci. 7, 1620-1625. [PDF]
Selwood, T., McCaslin, D. R., and Schechter, N. M. (1998) Spontaneous inactivation of human tryptase involves conformational changes consistent with conversion of the active site to a zymogen-like structure. Biochemistry 37, 13174-13183. [PDF]
Stanger, H. E., and Gellman, S. H. (1998) Rules for antiparallel beta-sheet design: D-Pro – Gly is superior to L-Asn – Gly for beta-hairpin nucleation. J. Am. Chem. Soc. 120, 4236-4237. [PDF]
1997
Blehert, D. S., Knoke, K. L., Fox, B. G., and Chambliss, G. H. (1997) Regioselectivity of nitroglycerin denitration by flavoprotein nitroester reductases purified from two Pseudomonas species. J. Bacteriol. 179, 6912-6920. [PDF]
Haque, T. S., and Gellman, S. H. (1997) Insights on beta-hairpin stability in aqueous solution from peptides with enforced type 1′ and type II’ beta-turns. J. Am. Chem. Soc. 119, 2302-2304. [PDF]
Selzer, R. R., and Elfarra, A. A. (1997) Chemical modification of deoxycytidine at different sites yields adducts of different stabilities: characterization of N3- and O2-deoxycytidine and N3-deoxyuridine adducts of butadiene monoxide. Arch. Biochem. Biophys. 343, 63-72. [PDF]
1996
Schenck, H. L., Dado, G. P., and Gellman, S. H. (1996) Redox-triggered secondary structure changes in the aggregated states of a designed methionine-rich peptide. J. Am. Chem. Soc. 118, 12487-12494. [PDF]
Truckses, D. M., Somoza, J. R., Prehoda, K. E., Miller, S. C., and Markley, J. L. (1996) Coupling between trans/cis proline isomerization and protein stability in staphylococcal nuclease. Protein Sci. 5, 1907-1916. [PDF]