The Direct Detect is an infrared (IR)-based system capable of quantification of proteins, lipids and detergents. The analysis measures amide bonds in polypeptide chains and can accurately quantify protein concentration without relying on amino acid composition, dye binding properties or redox potential. The analysis is quick and requires only 2 µL of sample. Samples are spotted on a PTFE card and run on the Direct Detect system. Accurate measurement of protein concentration from 0.25 to 5 mg/mL.