Microplate Readers
The BIF acquired two Tecan microplate readers in Jan 2014. Either reader is available for walkup use, but can be reserved if scheduled runs are needed. If you have a sample that is time sensitive, it is best to reserve time to make sure that your sample can be run efficiently. All users must be trained by BIF staff. To request training, contact Dan Stevens.
Documentation and Application Notes
Tecan infinite M1000 Pro
The M1000 Pro is a monochromator based instrument capable of measurement of absorbance (single point or scan), fluorescence (single point or excitation/emission scans), fluorescence polarization, luminescence, luminescence dual color, luminescence scan and AlphaScreen/AlphaLISA. The M1000 can heat samples (but cannot cool below ambient temperatures) and can shake between reads.
The M1000 is compatible with 6-, 12-, 24-, 48-, 96-, 384-, 1536-well plates. It is also possible to program in a custom plate layout.
The M1000 is equipped with an injection module with dual syringe pumps. These pumps are capable of liquid
delivery into 6- to 384-well plates which can be done independently or as part of a measurement protocol.
Tecan infinite F500
The Tecan F500 is a filter based instrument capable of absorbance, fluorescence intensity, fluorescence polarization, luminescence and dual color luminescence. The instrument can shake and take periodic measurements while regulating temperature (above ambient) to monitor reactions or growth curves.
The current filters available for use are listed below. If there is a filter that might help your research, contact BIF staff to.
| Measurement Mode | Excitation Filter (Bandwidth) | Measurement Mode | Emission Filter (Bandwidth) |
| Fluorescence Intensity | 320 nm (25 nm) | Fluorescence Intensity | 620 nm (10 nm) |
| Fluorescence Intensity | 485 nm (20 nm) | Fluorescence Intensity | 665 nm (8 nm) |
| Absorbance | 492 nm (10 nm) | Fluorescence Intensity | 535 nm (25 nm) |
| Fluorescence Intensity | 340 nm (35 nm) | Fluorescence Intensity | 612 nm (10 nm) |
| Fluorescence Intensity | 612 nm (10 nm) | Fluorescence Intensity | 670 nm (25 nm) |
| Fluorescence Intensity | 535 nm (25 nm) | Fluorescence Intensity | 590 nm (20 nm) |
| Fluorescence Polarization | 485 nm (20 nm) – Polarized | Fluorescence Polarization | 535 nm (25 nm) – Polarized* |
| Absorbance | 600 nm (10 nm) | Fluorescence Intensity | 510 nm (10 nm) |
| Fluorescence Intensity | 465 nm (20 nm) | – | – |
*There are 2 identical polarized emission filters that are perpendicular to each other for fluorescence polarization measurements.
The F500 is compatible with 6-, 12-, 24-, 48-, 96-, 384-, 1536-well plates. It is also possible to program in a custom plate layout.
NanoQuant Plate
Similar to a NanoDrop/NanoVue, the NanoQuant plate is a custom plate for use in determining the amount of nucleic acids in very small samples (2uL) with up to 16 samples on a single plate.