Microplate Readers
The BIF has two microplate readers acquired in 2024. Either reader is available for walkup use, but can be reserved if scheduled runs are needed. If you have a sample that is time sensitive, it is best to reserve time to make sure that your sample can be run efficiently. All users must be trained by BIF staff. To request training, contact Dan Stevens.
The Spark has fusion optics and uses either monochromators or filters and is capable of measurement of absorbance (single point or scan), fluorescence (single point or excitation/emission scans), fluorescence polarization, luminescence, luminescence dual color, luminescence scan and AlphaScreen/AlphaLISA. The Spark can heat or cool samples and can shake between reads. Polarization/Anisotropy can be carried out using either monochromators or filters and have wide wavelength selections.
The Spark is compatible with 6-, 12-, 24-, 48-, 96-, 384-, 1536-well plates. It is also possible to program in a custom plate layout.
The Spark is equipped with an injection module with dual syringe pumps. These pumps are capable of liquid delivery into 6- to 384-well plates which can be done independently or as part of a measurement protocol.
With similar capabilities to the Tecan Spark, the CLARIOstar Plus is equipped with LVF (Linear Variable Filter) monochromator technology as well as filters for many options for absorbance or fluorescence reads. The instrument has excellent sensitivity and is ideal for fluorescence polarization/anisotropy measurements. It also can measure luminescence (scan, single- or double-point) and is compatible with a range of plate types (from 6- to 1536-wells).
NanoQuant Plate
Similar to a NanoDrop/NanoVue, the NanoQuant plate is a custom plate for use in determining the amount of nucleic acids in very small samples (2uL) with up to 16 samples on a single plate.
Documentation and Application Notes
Tecan Spark Calendar
CLARIOstar Calendar
Public view Tecan Spark Calendar
Public view CLARIOstar Calendar